Abstract
There has been increased attention given to polymeric nanoparticles as protein carriers. In this regard, chitosan/tripolyphosphate (TPP) nanoparticles are considered to be a simple and efficient carrier. However, to have an ideal protein release profile, we need to optimize the properties of the carrier.
This study examined the influence of 4 critical process parameters on the physicochemical characteristics of final nanoparticles. Chitosan-based nanoparticles were produced by ionic gelation, and then the size, polydispersity and zeta potential of those resulting nanoparticles were evaluated. Subsequently, the encapsulation efficiency of bovine serum albumin as model protein was investigated.
The morphologies of nanoparticles were characterized using field emission scanning electron microscopy (FE-SEM). Linear mathematical models were presented for each response through 3 levels using Central Composite Design with the help of design of experiments software, and formulation optimization was performed.
Such research will serve as a basic study in protein loading into TPP cross-linked chitosan nanoparticles.
J Appl Biomater Funct Mater 2016; 14(4): e413 - e422
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/jabfm.5000278
Authors
Nasim Kiaie, Rouhollah M. Aghdam, Seyed H.A. Tafti, Shahriar H. EmamiArticle History
- • Accepted on 19/01/2016
- • Available online on 01/09/2016
- • Published online on 02/11/2016
Disclosures
This article is available as full text PDF.
Authors
- Kiaie, Nasim [PubMed] [Google Scholar] 1
- Aghdam, Rouhollah M. [PubMed] [Google Scholar] 2, 3, * Corresponding Author ([email protected],)
- Tafti, Seyed H.A. [PubMed] [Google Scholar] 2, * Corresponding Author ([email protected],)
- Emami, Shahriar H. [PubMed] [Google Scholar] 1
Affiliations
-
Department of Tissue Engineering, Amirkabir University of Technology, Tehran - Iran -
Biomedical Engineering Faculty, Islamic Azad University, Science and Research Branch, Tehran - Iran -
Tehran Heart Hospital Research Center, Tehran University of Medical Sciences, Tehran - Iran
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