Abstract
Tissue engineering is a field which is currently under a great deal of investigation for the development and/or restoration of tissue and organs, through the combination of cell therapy with biomaterials. Rapid prototyping or additive manufacturing is a versatile technology which makes possible the fabrication of three dimensional (3D) structures from a wide range of materials with complex geometry and accuracy, such as scaffolds.
The aim of this study has been to investigate the interaction between mesenchymal stem cells with poly (ε-caprolactone) (PCL) biomaterials used for obtaining scaffolds through additive manufacturing.
Scanning electron microscopy, confocal microscopy and biological assays were performed to analyse the successful interaction between the cells and the biomaterials.
As a result, the number of viable cells attached to the scaffolds was lower when compared to the control group; however, it was possible to observe cells in the scaffolds since day 1 of analysis, with regions of confluence after 21 days of seeding.
To conclude, these biomaterials are interesting if used as medical artifacts, principally in tissue with prolonged regeneration time and which requires 3D supports with good mechanical properties.
J Appl Biomater Funct Mater 2016; 14(1): e19 - e25
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/jabfm.5000252
Authors
Daniela Steffens, Rodrigo Alvarenga Rezende, Bruna Santi, Frederico David Alencar de Sena Pereira, Paulo Inforçatti Neto, Jorge Vicente Lopes da Silva, Patricia PrankeArticle History
- • Accepted on 31/05/2015
- • Available online on 04/12/2015
- • Published online on 06/04/2016
Disclosures
This article is available as full text PDF.
Authors
- Steffens, Daniela [PubMed] [Google Scholar] 1, 2, * Corresponding Author ([email protected])
- Alvarenga Rezende, Rodrigo [PubMed] [Google Scholar] 3
- Santi, Bruna [PubMed] [Google Scholar] 2
- Alencar de Sena Pereira, Frederico David [PubMed] [Google Scholar] 3
- Inforçatti Neto, Paulo [PubMed] [Google Scholar] 3
- Lopes da Silva, Jorge Vicente [PubMed] [Google Scholar] 3
- Pranke, Patricia [PubMed] [Google Scholar] 1, 2, 4
Affiliations
-
Post-graduation Program of Biological Science – Physiology, Federal University of Rio Grande do Sul, UFRGS, Porto Alegre, Rio Grande do Sul - Brazil -
Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Federal University of Rio Grande do Sul, UFRGS, Porto Alegre, Rio Grande do Sul - Brazil -
Division of 3D Technologies, Center for Information Technology Renato Archer (CTI), Campinas, São Paulo - Brazil -
Stem Cell Research Institute, Porto Alegre, Rio Grande do Sul - Brazil
Article usage statistics
The blue line displays unique views in the time frame indicated.
The yellow line displays unique downloads.
Views and downloads are counted only once per session.