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In situ development of nanosilver-impregnated bacterial cellulose for sustainable released antimicrobial wound dressing

Abstract

Purpose

Bacterial cellulose (BC) is an interesting biomaterial found application in various fields due to its novel characteristics like purity, water holding capacity, degree of polymerization and mechanical strength. BC as wound dressing material has limitation because it has no antimicrobial activity. To circumvent this problem, the present study was carried out by impregnation of silver on bacterial cellulose surface.

Methods

Bacterial cellulose was produced by Gluconoacetobacter hansenii (strain NCIM 2529) by shaking culture method. The sodium borohydride and classical Tollens reaction was used for silver nanoparticle synthesis.

Results

The effectiveness of sodium borohydride method compared with Tollens reaction was evaluated on the basis of silver nanoparticle formation and its impregnation on BC as evidenced by UV-Vis spectrum analysis, FE-SEM-EDS analysis and FT-IR spectrum. The potential of nano silver impregnated BC was determined for sustained release antimicrobial wound dressing material by swelling ratio, mechanical properties and antimicrobial activity against Staphylococcus aureus.

Conclusions

Thus the nanosilver impregnated bacterial cellulose as promising antimicrobial wound dressing material was evidenced.

J Appl Biomater Funct Mater 2016; 14(1): e53 - e58

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000257

Authors

Bhavana V. Mohite, Satish V. Patil

Article History

Disclosures

Financial support: Author (BVM) is thankful to UGC BSR (F.4-1/2006 (BSR)/7-137/2007), New Delhi, India for financial assistance for the work. Authors are grateful to UGC and DST for making the research facilities available under the UGC-SAP and DST-FIST programmes sanctioned to the School of Life Sciences.
Conflict of interest: The authors have no conflicts of interest to declare.

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Authors

Affiliations

  • School of Life Sciences, North Maharashtra University, Jalagoan, Maharastra - India

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