Abstract
In this work, chitosan (CH) was used to produce a novel coating for Ti6Al4V, the most widely used alloy in orthopedic implants, so as to improve the biological tissue response at the metallic surface.
The Ti6Al4V surface was sandblasted with alumina particles. CH was chemically modified, via carbodiimide chemistry, using lactobionic and 4-azidebenzoic acid to make it soluble at physiological pH and photocrosslinkable, respectively. The reaction was verified by FTIR, NMR and UV/vis spectroscopy. Ti6Al4V surfaces were coated with solutions of the modified CH and exposed to UV light, causing polymer crosslinking and formation of a hydrogel on the surface. The crosslinking reaction was monitored by FTIR at different exposure times. Coating morphology was observed by SEM. The coating´s cytocompatibility was determined in vitro through the culture of rat bone marrow mesenchymal stem cells, using an MTT assay, with their morphology assessed by SEM.
The developed coating behaved as a hydrogel on the Ti6Al4V and was stable on the surface. FTIR and NMR confirmed the crosslinking mechanism, based on an arile ring expansion, and subsequent reaction with the CH amine groups. Furthermore, the coating was able to support cell proliferation and osteogenic differentiation.
UV crosslinking of CH is easy to apply and has potential for future metallic implant surface modifications. Due to its nature as a hydrogel, the coating could be used for further studies in the encapsulation of bioactive molecules to improve osteogenic potential at the tissue–implant interface.
J Appl Biomater Funct Mater 2015; 13(3): e210 - e219
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/jabfm.5000227
Authors
Denise Zujur, Josnell Moret, Dubrasvka Rodriguez, Lauren Cruz, Joaquin Lira, Linda Gil, Ernic Dominguez, Jose Alvarez-BarretoArticle History
- • Accepted on 10/01/2015
- • Available online on 12/06/2015
- • Published online on 16/10/2015
Disclosures
This article is available as full text PDF.
Authors
- Zujur, Denise [PubMed] [Google Scholar] 1, 2
- Moret, Josnell [PubMed] [Google Scholar] 1
- Rodriguez, Dubrasvka [PubMed] [Google Scholar] 1
- Cruz, Lauren [PubMed] [Google Scholar] 1
- Lira, Joaquin [PubMed] [Google Scholar] 2
- Gil, Linda [PubMed] [Google Scholar] 3
- Dominguez, Ernic [PubMed] [Google Scholar] 4
- Alvarez-Barreto, Jose [PubMed] [Google Scholar] 1, 5, * Corresponding Author ([email protected])
Affiliations
-
Human Tissue Engineering Laboratory, Health Sciences, Institute of Advanced Studies (IDEA), Sartenejas Valley, Caracas - Venezuela -
Department of Materials Science, Simon Bolivar University, Sartenejas Valley, Caracas - Venezuela -
Center for Biomaterials and Corrosion, National Experimental University “Antonio Jose de Sucre” (UNEXPO), Puerto Ordaz, Bolivar State - Venezuela -
Department of Traumatology, Uyapar Hospital, Puerto Ordaz, Bolivar State - Venezuela -
State University of Milagro, National Secretary of Higher Education, Science and Technology, Prometeo Project - Ecuador
Article usage statistics
The blue line displays unique views in the time frame indicated.
The yellow line displays unique downloads.
Views and downloads are counted only once per session.