Advertisement
< Vol. 12 Issue 3 | Sep-Dec 2014 | pp. 113 - 285

Adherence of S. epidermidis on different metals. A comparative in vitro study

Abstract

Aim

Staphylococcus epidermidis is the most common cause of orthopedic infections. Adhesion and biofilm formation on orthopedic implant surfaces play an important role in the physiopathology of these infections. The aim of our study was to evaluate the adhesion of S. epidermidis on the surface of metals usually used in orthopedics.

 Methods

Previously sterilized circular metal plates of titanium (Ti), porous titanium (p-Ti), cobalt chromium (CoCr) and stainless steel (SS) were hung completely submerged in a liquid medium with a known concentration of S. epidermidis (RP62A). They were incubated for 1 h or 24 h at 36°C. After incubation, each plate was washed with PBS and sonicated during 5 minutes in 10 mL of saline. Different dilutions were performed and 100 µL from each sample was cultured on agar plates.

 Results

26 metal plates were incubated for 1 h and other 55 metal plates for 24 h. The lowest bacterial count (cfu/mm2) at 1 h was observed in CoCr plates while in p-Ti it was 6 times higher. At 24 h the highest bacterial count was observed in SS plates while the lowest in Ti. However, these differences were not statistically significant.

 Conclusions

After 1 h and 24 h of exposure, the lowest adherence was observed in CoCr and Ti plates, respectively. However, bacterial attachment occurred with all materials. It is necessary to further investigate new materials able to avoid bacterial attachment.

J Appl Biomater Funct Mater 2014; 12(3): 141 - 144

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000210

Authors

Juan Castellanos, Araceli González-Cuevas, Josep M. Sierra, Laura García-Nuño, Vicenç Diaz-Brito, Alex Soriano, Lluís Font-Vizcarra

Article History

  • • Accepted on 19/12/2013
  • • Available online on 21/07/2014
  • • Published online on 30/12/2014

Disclosures

Financial support: This study was partially supported by a grant from Parc Sanitari Sant Joan de Deu and Mutual Medica. The production cost of the metal plates was supported by AMES.
Conflict of interest: None.

This article is available as full text PDF.

  • If you are a Subscriber, please log in now.

  • Article price: Eur 36,00
  • You will be granted access to the article for 72 hours and you will be able to download any format (PDF or ePUB). The article will be available in your login area under "My PayPerView". You will need to register a new account (unless you already own an account with this journal), and you will be guided through our online shop. Online purchases are paid by Credit Card through PayPal.
  • If you are not a Subscriber you may:
  • Subscribe to this journal
  • Unlimited access to all our archives, 24 hour a day, every day of the week.

Authors

Affiliations

  • Department of Trauma and Orthopedics, Parc Sanitari Sant Joan de Deu, Sant Boi de Llobregat, Barcelona - Spain
  • Department of Microbiology, Parc Sanitari Sant Joan de Deu, Sant Boi de Llobregat, Barcelona - Spain
  • Department of Microbiology, Hospital de Bellvitge, Barcelona - Spain
  • Department of Internal Medicine and Infectious Disease, Parc Sanitari Sant Joan de Deu, Sant Boi de Llobregat, Barcelona - Spain
  • Department of Infectious Disease, Hospital Clínic, Barcelona - Spain

Article usage statistics

The blue line displays unique views in the time frame indicated.
The yellow line displays unique downloads.
Views and downloads are counted only once per session.

No supplementary material is available for this article.