Abstract
In this study, osteoblast-like MG-63 cells were cultured on 3 different scaffold types composed of (a) collagen + poly-L-lactic acid (PLLA), (b) collagen + hydroxyapatite (HA; 30ºC) or (c) collagen + hydroxyapatite (HA; 37ºC) and produced with different porosities.
Biomechanical properties of the scaffolds were characterized by tensile strength measurements. Properties of the cell-seeded scaffolds were evaluated with scanning electron microscopy (SEM). Cell adhesion and proliferation capacities were evaluated. Alkaline phosphatase (ALP) levels in media were measured. Transmission electron microscopy (TEM) and histological analyses were used to assess morphological characteristics.
Our results showed that collagen-based PLLA and HA scaffolds have good cell biocompatibility. MTT test showed that the scaffolds exhibited no cytotoxicity. According to the force and displacement data, collagen + HA at 37ºC showed the highest mechanical strength and displacement.
The results suggest that collagen-based PLLA and HA scaffolds might improve osteoblastic growth in vitro and have biomaterial integration potential in possible therapeutic approaches for future clinical studies.
J Appl Biomater Funct Mater 2015; 13(1): e10 - e16
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/jabfm.5000182
Authors
Berivan Cecen, Didem Kozaci, Mithat Yuksel, Diler Erdemli, Alper Bagriyanik, Hasan HavitciogluArticle History
- • Accepted on 24/06/2013
- • Available online on 11/04/2014
- • Published online on 18/03/2015
Disclosures
This article is available as full text PDF.
Authors
- Cecen, Berivan [PubMed] [Google Scholar] 1, * Corresponding Author ([email protected])
- Kozaci, Didem [PubMed] [Google Scholar] 2
- Yuksel, Mithat [PubMed] [Google Scholar] 3
- Erdemli, Diler [PubMed] [Google Scholar] 1
- Bagriyanik, Alper [PubMed] [Google Scholar] 4
- Havitcioglu, Hasan [PubMed] [Google Scholar] 1, 5
Affiliations
-
Department of Biomechanics, The Institute of Health Science, Dokuz Eylul University, Izmir - Turkey -
Department of Medical Biochemistry, Medical Faculty, Adnan Menderes University, Aydin - Turkey -
Department of Chemistry Engineering, Engineering Faculty, Ege University, Izmir - Turkey -
Department of Histology, Medicine Faculty, Dokuz Eylul University, Izmir - Turkey -
Department of Orthopaedics and Traumatology, Medicine Faculty, Dokuz Eylul University, Izmir - Turkey
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