Abstract
Purpose: When investigating apatite formation on biomaterial surfaces, simulated body fluid (SBF) is used as an in vitro solution, however, it does not provide an appropriate environment for the growth of bacterial biofilm. The aim of the present study was to compare the bioactivity in terms of apatite formation on two bioactive glass (BAG) composite surfaces using both SBF and bacterial-biofilm growing medium (BM).
Methods: Polymer composite substrates with different percentages of BAG–particles (50% and 75% by weight) were prepared. Plain resin substrates were used as a negative control. The substrates were immersed in SBF and BM for 3 days. The surface and, subsequently, the cross-sections of the substrates were examined with scanning electron microscopy (SEM) and x-ray energy dispersive spectroscopy (EDS).
Results: All the investigated BAG-composite surfaces showed apatite formation after immersion in SBF and BM liquid media.
Conclusions: The use of BM is a promising method for studies involving simultaneous biofilm growth and apatite formation on bioactive surfaces.
J Appl Biomater Funct Mater 2013; 11(2): 95 - 98
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/JABFM.5000154
Authors
Julia Kulkova, Aous A. Abdulmajeed, Eija Könönen, Timo O. Närhi
Article History
- • Accepted on 24/11/2012
- • Available online on 31/05/2013
- • Published online on 13/09/2013
This article is available as full text PDF.
Authors
- Kulkova, Julia
[PubMed]
[Google Scholar]
Department of Periodontology, Institute of Dentistry, University of Turku, Turku - Finland and Turku Clinical Biomaterials Centre - TCBC, University of Turku, Turku - Finland
- Abdulmajeed, Aous A.
[PubMed]
[Google Scholar]
Department of Prosthetic Dentistry, Institute of Dentistry, University of Turku, Turku - Finland and Finnish Doctoral Program in Oral Sciences - FINDOS, Institute of Dentistry, University of Turku, Turku - Finland and Turku Clinical Biomaterials Centre - TCBC, University of Turku, Turku - Finland
- Könönen, Eija
[PubMed]
[Google Scholar]
Department of Periodontology, Institute of Dentistry, University of Turku, Turku - Finland
- Närhi, Timo O.
[PubMed]
[Google Scholar]
Department of Prosthetic Dentistry, Institute of Dentistry, University of Turku, Turku - Finland and Turku Clinical Biomaterials Centre - TCBC, University of Turku, Turku - Finland and Clinic of Oral Diseases, Turku University Central Hospital, Turku - Finland
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