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< Vol. 14 Issue 4 | Oct-Dec 2016 | pp. e395 - e501

Design and cytocompatibility of chitosan-based thermoresponsive cell culture plates

Abstract

Background

The aim of this study was to develop a novel thermoresponsive material suited for tissue engineering and investigate the growth and harmless detachment of cells cultured on the surface of thermoresponsive tissue culture polystyrene (TCPS).

 Methods

Thermoresponsive N-isopropylacrylamide (NIPAAm) and biocompatible chitosan (CS) were grafted onto the surface of TCPS by ultraviolet (UV)–induced graft polymerization. The chemical composition, surface morphology and thermoresponsiveness of the modified TCPS were investigated by X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM) and contact angle (CA), respectively. Furthermore, the growth and detachment behaviors of mouse fibroblast cells (L929) on the surface of the modified TCPS were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

 Results

The modified TCPS exhibited good hydrophobic/hydrophilic property alterations in response to temperature. The cytocompatibility of the materials was improved due to the introduction of CS. Cells could be spontaneously detached from the surface without any damage, by controlling environmental temperature. The viability of cells obtained by temperature induction was higher than that obtained by enzymatic digestion.

 Conclusions

This study developed a simple and economical method to fabricate thermoresponsive cell culture dishes and provided new thoughts and experimental bases for exploring novel material applied in tissue engineering.

J Appl Biomater Funct Mater 2016; 14(4): e404 - e412

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000276

Authors

Xiaoling He, Yuxin Zhao, Zhenli Jin, Yu Su, Huiqin An, Lili Ge, Dongsheng Wei, Li Chen

Article History

  • • Accepted on 20/01/2016
  • • Available online on 30/05/2016
  • • Published online on 02/11/2016

Disclosures

Financial support: This work was supported by Science and Technology Correspondent Project of Tianjin (Grant No. 15JCTPJC57500), Natural Science Foundation of Tianjin (Grant No. 16JCYBJC23800) and National Natural Science Foundation of China (Grant No. 51303129).
Conflict of interest: None of the authors has any financial interest related to this study to disclose.

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Authors

Affiliations

  • School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin - PR China
  • Tianjin Key Laboratory of Fiber Modification and Functional Fiber, Tianjin Polytechnic University, Tianjin - PR China
  • College of Life Sciences, Nankai University, Tianjin - PR China

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