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< Vol. 14 Issue 2 | Apr-Jun 2016 | pp. e129 - e222

Role of prostaglandin E2 in the modulation of Wnt canonical signaling in cells on microstructured titanium surfaces

Abstract

Background

Rough surface topography enhances the activation of Wnt canonical signaling, a pathway required for osteoblast differentiation. The present study investigated the effects of the modulation of prostaglandin E2 (PGE2) signaling on osteoblastic differentiation on titanium surfaces for endosseous implants with different topographies.

 Methods

C2C12 cells were plated on polished or acid-etched/sand-blasted (SLA) titanium discs and stimulated with 1 μM PGE2 or 100 nM cyclooxygenase inhibitor indomethacin. Activation of Wnt canonical signaling was measured with a reporter system. Gene expression was measured in the same cell system by real-time polymerase chain reaction (RT-PCR). Osteoblastic MC3T3 cells were then plated on polished or SLA titanium discs with or without indomethacin, and their proliferation and the expression of osteoblast-specific genes was assessed by RT-PCR. Cell morphology was furthermore studied on SEM, and cell adhesion was assessed by fluorescent labeling of focal adhesion.

 Results

PGE2 decreased Wnt signaling stimulation in cells growing on polished or SLA surfaces, while indomethacin increased the expression of Wnt target genes in C2C12 and MC3T3 cells, by reporter assay. Moreover, indomethacin increased the expression of early differentiation marker alkaline phosphatase in MC3T3 cells on polished discs and of late marker osteocalcin in cells on SLA titanium.

 Conclusions

Prostaglandin signaling affects the activation of Wnt canonical pathway in osteoblastic and mesenchymal cells on microstructured surfaces.

J Appl Biomater Funct Mater 2016; 14(2): e181 - e188

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000267

Authors

Edoardo Manfredi, Simone Lumetti, Federico Rivara, Andrea Toffoli, Elena Calciolari, Antonio Cacchioli, Francesca Ravanetti, Giulia Ghiacci, Guido Macaluso, Carlo Galli

Article History

  • • Accepted on 12/12/2015
  • • Available online on 06/05/2016
  • • Published online on 18/05/2016

Disclosures

Financial support: The study was funded by grant 839_2012 from the ITI Foundation (Basel, Switzerland) and FIL research grant from the University of Parma.
Conflict of interest: The authors have no conflicts of interest to disclose.

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Authors

Affiliations

  •  Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Parma - Italy
  •  Centro Universitario di Odontoiatria, Dental School, University of Parma, Parma - Italy
  •  Department Veterinary Sciences, University of Parma, Parma - Italy
  •  Institute for Electronics and Magnetism IMEM-CNR, Parma - Italy

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