Abstract
Silver ions (Ag+) have strong antibacterial effects, and silver-coated materials are in widespread clinical use. However, the application of silver-coated medical devices is not without concerns: its use with direct bone contact is not established, and systemic toxic side effects of released Ag+ have been described. Therefore, alternative bactericidal coatings with a more localized way of acting – e.g., calcium dihydroxide, Ca(OH)2 (CH) – would be advantageous.
A new rat model of the animal’s tibial metaphysis was developed. In the left proximal tibiae of 36 male Wistar rats, titanium screws were implanted. The screws were coated with hydroxyapatite (HA; 12 animals: group I), low-dosed HA silver (HA-Ag; 12 animals: group II) and CH (12 animals: group III). After 6 weeks, all rats were sacrificed. The implants were evaluated for morphological changes on their surfaces, by light microscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy; for osteointegration, by measurement of resistance to removal; and for bacterial colonization, by quantitative culture analysis. Additionally, the tibial bone was investigated histologically for signs of osteomyelitis and sonicated to detect bacterial loads.
(i) No microbiological or histological signs of infection could be determined on any of the screws or the surrounding bone. (ii) The bone-implant interface analysis revealed extensive bone formation and direct bone-implant contact on all HA, HA-Ag and HA-CH coated screws. (iii) HA and HA-Ag were partially, and CH was fully, degraded on the screw coating, allowing host bone to osteointegrate.
J Appl Biomater Funct Mater 2016; 14(4): e441 - e448
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/jabfm.5000323
Authors
Norbert Harrasser, Michael de Wild, Johannes Gorkotte, Andreas Obermeier, Susanne Feihl, Melanie Straub, Ruediger von Eisenhart-Rothe, Hans Gollwitzer, Jasmine Rüegg, Walter Moser, Philipp Gruner, Rainer BurgkartArticle History
- • Accepted on 15/06/2016
- • Available online on 17/09/2016
- • Published online on 02/11/2016
Disclosures
This article is available as full text PDF.
Authors
- Harrasser, Norbert [PubMed] [Google Scholar] 1, * Corresponding Author ([email protected])
- de Wild, Michael [PubMed] [Google Scholar] 2
- Gorkotte, Johannes [PubMed] [Google Scholar] 1
- Obermeier, Andreas [PubMed] [Google Scholar] 1
- Feihl, Susanne [PubMed] [Google Scholar] 3
- Straub, Melanie [PubMed] [Google Scholar] 4
- von Eisenhart-Rothe, Ruediger [PubMed] [Google Scholar] 1
- Gollwitzer, Hans [PubMed] [Google Scholar] 1, 5
- Rüegg, Jasmine [PubMed] [Google Scholar] 2
- Moser, Walter [PubMed] [Google Scholar] 6
- Gruner, Philipp [PubMed] [Google Scholar] 7
- Burgkart, Rainer [PubMed] [Google Scholar] 1
Affiliations
-
Clinic of Orthopedics and Sports Orthopedics, Klinikum rechts der Isar, Technical University of Munich, Munich - Germany -
University of Applied Sciences and Arts Northwestern Switzerland (FHNW), School of Life Sciences, Muttenz - Switzerland -
Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Munich - Germany -
Institute of Pathology, Klinikum rechts der Isar, Technical University of Munich, Munich - Germany -
ATOS Clinic, Munich - Germany -
Atesos Medical AG, Aarau - Switzerland -
Medicoat AG, Maegenwil - Switzerland
Article usage statistics
The blue line displays unique views in the time frame indicated.
The yellow line displays unique downloads.
Views and downloads are counted only once per session.