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Hydroxyapatite decreases cytotoxicity of a glass ionomer cement by calcium fluoride uptake in vitro

Abstract

Background

Glass ionomer cements (GICs) are widely used in dentistry because of their remineralizing and cariostatic potential induced by fluoride. In vitro studies have reported cell toxicity triggered by GICs; however, the influence of hydroxyapatite (HAp) must be considered. The aim of this study was to evaluate the effect of HAp in decreasing the cytotoxicity of the GIC 3M Vitrebond in vitro.

Methods

Samples of 3M Vitrebond (powder, liquid and light-cured) were incubated in Dulbecco’s modified Eagle’s medium–Ham’s F12 (DMEM-F12) for 24 hours at 37°C. Subsequently, the light-cured medium was treated with 100 mg/mL of HAp overnight. Toxicity of conditioned media diluted 1:2, 1:4, 1:8 and 1:20 was analyzed on human gingival fibroblasts (HGFs) using light microscopy and the fluorometric microculture cytotoxicity assay. The amounts of calcium fluoride (CaF2) were determined by the alizarin red S method.

Results

The exposure of HGFs to light-cured induced cell death and morphological changes such as chromatin condensation, pyknotic nuclei and cytoplasmic modifications. Exposure to light-cured treated with HAp, significantly increased cell viability leading to mostly spindle-shaped cells (p<0.001). The concentration of CaF2 released by the light-cured was 200 ppm, although, in the light-cured/HAp conditioned medium, this quantity decreased to 88 ppm (p<0.01).

Conclusions

These data suggest that HAp plays a protective role, decreasing the cytotoxic effect of 3M Vitrebond induced by CaF2.

Post author correction

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000381

Authors

Casiano Del Angel-Mosqueda, Rene Hernandez-Delgadillo, Osvelia E. Rodríguez-Luis, María T. Ramírez-Rodríguez, Silvia Munguía-Moreno, Norma V. Zavala-Alonso, Juan M. Solís-Soto, María A. A. Nakagoshi-Cepeda, Rosa I. Sánchez-Nájera, Sergio E. Nakagoshi-Cepeda, Claudio Cabral-Romero

Article History

Disclosures

Financial support: Funding was received from the CONACyT to finance this project (grant no. 183825).
Conflict of interest: The authors declare that they have no conflicts of interest.

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Authors

Affiliations

  • Autonomous University of Nuevo Leon, Faculty of Dentistry, Monterrey, Nuevo Leon - Mexico
  • Autonomous University of San Luis Potosi, Faculty of Stomatology, San Luis Potosi - Mexico

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