Abstract
Infections of implants pose a severe problem in the field of orthopedic surgery, because they can cause bone degradation with subsequent loosening of the implant. The discrimination between septic implant loosening and aseptic loosening can be a challenge, and hence novel diagnostic methods have been introduced to improve the detection of bacteria. Because a major problem is their firm adherence to implants due to biofilm formation, sonication has been introduced, followed by identification of bacteria by culture or genetic methods. In this study, we compared the results obtained after sonication pretreatment with those of microbiological testing of tissue samples and histopathological evaluation of the same tissue. Furthermore, we related the results obtained following sonication to the clinical diagnosis of septic or aseptic implant loosening, respectively. Sonication of explanted devices also enhances the likelihood of detecting bacterial growth in patients who were considered “aseptic” based on the clinical evaluation.
J Appl Biomater Funct Mater 2014; 12(3): 135 - 140
Article Type: ORIGINAL RESEARCH ARTICLE
DOI:10.5301/jabfm.5000205
Authors
Ulrike Dapunt, Burkhard Lehner, Irene Burckhardt, Stefan Zimmermann, Gertrud M. Hänsch, Volker EwerbeckArticle History
- • Accepted on 12/12/2013
- • Available online on 06/09/2014
- • Published online on 30/12/2014
Disclosures
This article is available as full text PDF.
Authors
- Dapunt, Ulrike [PubMed] [Google Scholar] 1, * Corresponding Author ([email protected])
- Lehner, Burkhard [PubMed] [Google Scholar] 1
- Burckhardt, Irene [PubMed] [Google Scholar] 2
- Zimmermann, Stefan [PubMed] [Google Scholar] 2
- Hänsch, Gertrud M. [PubMed] [Google Scholar] 3
- Ewerbeck, Volker [PubMed] [Google Scholar] 1
Affiliations
-
Department for Orthopedics, Traumatology and Paraplegiology, University of Heidelberg, Heidelberg - Germany -
Department for Infectious Diseases, Medical Microbiology and Hygiene, University of Heidelberg, Heidelberg - Germany -
Department of Immunology, University of Heidelberg, Heidelberg - Germany -
Ulrike Dapunt and Burkhard Lehner contributed equally.
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