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< Vol. 13 Issue 2 | Apr-Jun 2015 | pp. e73 - e193

Human plasma protein adsorption onto alumina nanoparticles relevant to orthopedic wear

Abstract

Purpose

Wear of ceramic orthopedic devices generates nanoparticles in vivo that may present a different biological character from the monolithic ceramic from which they are formed. The current work investigated protein adsorption from human plasma on alumina nanoparticles and monolithic samples representative of both wear particles and the ceramic components as implanted.

 Materials and methods

A physicochemical characterization of the particles and their dispersion state was carried out, and the protein adsorption profiles were analyzed using 1D SDS-PAGE and mass spectrometry.

 Results

Significant differences in protein-binding profiles were identified where the nanoparticles selectively bound known transporter proteins rather than the more highly abundant serum proteins that were observed on the monoliths.

 Conclusions

Proteins associated with opsonization of particles were seen to be present in the protein corona of the nanoparticles, which raises questions regarding the role of wear particles in periprosthetic tissue inflammation and aseptic loosening.

J Appl Biomater Funct Mater 2015; 13(2): e145 - e155

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000219

Authors

John P. Gibbons, Marco P. Monopoli, Martin Lundqvist, Iker Montes-Burgos, Eilis E. McGrath, Giuliano Elia, Iseult Lynch, Kenneth A. Dawson, Kenneth T. Stanton

Article History

  • • Accepted on 04/08/2014
  • • Available online on 22/05/2015
  • • Published online on 04/07/2015

Disclosures

Financial support: This work was supported by the European Union Sixth Framework Program project NanoInteract (grant no. NMP4-CT-2006-033231), the Irish Research Council for Science, Engineering and Technology (M.L.), SFI RFP 06/RFP/ CHP031 (I.M.B.) and an UCD Ad Astra scholarship (J.P.G.). Part of this work was conducted under the framework of the INSPIRE programme, funded by the Irish Government̕s Programme for Research in Third Level Institutions, Cycle 4, National Development Plan 2007-2013 (Éilis McGrath, M.P.M.).
Conflict of interest: The authors certify that they have no affiliations with, or involvement in, any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or ­patent-licensing arrangements), or any nonfinancial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

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Authors

Affiliations

  • UCD School of Mechanical and Materials Engineering, University College Dublin, Belfield, Dublin - Ireland
  • Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin - Ireland
  • Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Belfield, Dublin - Ireland

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