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< Vol. 10 Issue 3 | Sep-Dec 2012 | pp 163 - 314 - Focus on Advanced Functional Polymers for Medicine

Proliferation and osteoblastic differentiation of hMSCs on cellulose-based hydrogels

Abstract

Purpose: The aim of this project was to study the proliferation and differentiation of human Mesenchymal Stem Cells (hMSCs) onto a cellulose-based hydrogel for bone tissue engineering.
Methods: Modified-cellulose hydrogel was prepared via double esterification crosslinking using citric acid. The response of human Mesenchymal Stem Cells (hMSCs) in terms of cell proliferation and differentiation into osteoblastic phenotype was evaluated by using Alamar blue assay and Alkaline phosphatase activity. 
Results: The results showed that CMCNa and CMCNa_CA have no negative effect on hMSC, adhesion and proliferation. Moreover, the increase of the ALP expression for CMCNa_CA confirms the ability of the hydrogels to support the osteoblastic differentiation.
Conclusions: The cellulose-based hydrogels have a potential application as filler in bone tissue regeneration.

J Appl Biomater Funct Mater 2012; 10(3): 302 - 307

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/JABFM.2012.10366

Authors

Maria Grazia Raucci, Marco Antonio Alvarez-Perez, Christian Demitri, Alessandro Sannino, Luigi Ambrosio

Article History

  • • Accepted on 23/10/2012
  • • Available online on 13/12/2012
  • • Published in print on 13/02/2013

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Authors

  • Raucci, Maria Grazia [PubMed] [Google Scholar]
    Institute of Composite and Biomedical Materials, National Research Council of Italy, Naples - Italy
  • Alvarez-Perez, Marco Antonio [PubMed] [Google Scholar]
    Institute of Composite and Biomedical Materials, National Research Council of Italy, Naples - Italy
  • Demitri, Christian [PubMed] [Google Scholar]
    Department of Engineering for Innovation, University of Salento, Lecce - Italy
  • Sannino, Alessandro [PubMed] [Google Scholar]
    Department of Engineering for Innovation, University of Salento, Lecce - Italy
  • Ambrosio, Luigi [PubMed] [Google Scholar]
    Institute of Composite and Biomedical Materials, National Research Council of Italy, Naples - Italy

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