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Ultrastructural analysis of nanoparticles and ions released in periprosthetic membranes

Abstract

Purpose

The distribution and relationship of hydroxyapatite debris, nanometric organic and metal wear particles and metal ions on periimplant interface membranes following aseptic and septic arthroplastic loosening were investigated.

Methods

Scanning electron microscopy and X-ray spectroscopic analysis were used to analyze debris and ion distribution.

Results

Hydroxyapatite debris appeared with different morphology in a particular distribution among several membranes. These differences may reflect the occurrence of different friction forces taking place between prosthesis and bone interface in the several types of prostheses studied. Metal wear particles were detected in greater numbers in membranes from noncemented prostheses compared with those from cemented ones. In contrast, more organic particles were present in membrane from cemented prosthesis. No differences were observed between aseptic and septic membranes.

Conclusion

Our findings support the need to evaluate the occurrence of friction forces that periprosthetic bone debris production may induce to exacerbate cellular reactivity. Furthermore, cellular engulfment of debris and the high level of different ions released indicate the occurrence of a toxic environment that may induce failure of any reparative pathways.

J Appl Biomater Funct Mater 2014; 12(3): 210 - 217

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000183

Authors

Maurizio Sabbatini, Giorgio Gatti, Filippo Renò, Michela Bosetti, Leonardo Marchese, Mario Cannas

Article History

Disclosures

Financial support: This study was supported by authors’ own institution.
Conflict of interest: All authors declare no conflict of interest.

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Authors

Affiliations

  • Department of Health Science, Research Center of Biocompatibility and Tissue Engineering, University of Eastern Piedmont A. Avogadro, Alessandria, Novara, Vercelli - Italy
  • Interdisciplinary Centre of Science and Advanced Technology and Nano-Systems, University of Eastern Piedmont A. Avogadro, Alessandria, Novara, Vercelli - Italy
  • DISCAFF, Department of Human Anatomy, University of Eastern Piedmont A. Avogadro, Alessandria, Novara, Vercelli - Italy

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