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Preparation and characterization of gelatin/sericin/carboxymethyl chitosan medical tissue glue

Abstract

Background

The development and application of medical glue has been continuously expanding and advancing. However, there are few glues that combine low-cost with excellent biocompatibility.

Methods

We have prepared a medical tissue glue using a gelatin (Gel), sericin (SS) and carboxymethyl chitosan (CMCS) blend solution, cross-linked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC). The combination’s characteristics and microstructure morphology were observed by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). Bond strength tests were used to measure the bond strength of the glue. To assay blood compatibility, a hemolytic test, dynamic coagulation test and platelet adherence test were also investigated. Further, the cellular behavior of L-929 and a systemic acute toxicity test on the Gel/SS/CMCS tissue glue were also investigated by MTT and H&E staining.

Results

Characterization analysis showed that there was stable binding between raw materials, forming an amide bond with homogeneous holes. The bond strength of the tissue glue reached 2.50 ± 0.04 N in 10 minutes, slightly higher than the alpha-cyanoacrylate biological glue (2.25 ± 0.05 N). Blood compatibility tests revealed that the glue had outstanding blood compatibility. Further, cytotoxicity test and systemic acute toxicity test both showed that the glue was without cytotoxicity and not toxic to the body.

Conclusions

The Gel/SS/CMCS tissue glue we prepared at low cost had excellent biocompatibility and structural characteristics. It could be a better candidate for tissue engineering in biomedical applications applied in clinical practice to promote skin wound healing and to further reduce the formation of skin wound scars.

Post author correction

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/jabfm.5000384

Authors

Min Liang, Zhenzhu Li, Cengceng Gao, Fuping Wang, Zhongmin Chen

Article History

Disclosures

Financial support: Chongqing Science & Technology Commission Project (cstc2016shmszx0635).
Conflict of interest: None of the authors has any financial interest related to this study to disclose.

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Authors

Affiliations

  • School of Pharmacy and Biological Engineering, Chongqing University of Technology, Chongqing - PR China

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